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KMID : 1039120140030010100
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Clinical and Experimental Vaccine Research
2014 Volume.3 No. 1 p.100 ~ p.109
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Development of porcine respiratory and reproductive syndrome virus replicon vector for foot-and-mouth disease vaccine
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Jeeva Subbiah
Lee Jung-Ah
Park Seung-Yong
Song Chang-Seon
Choi In-Soo
Lee Joong-Bok
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Abstract
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Purpose: Foot-and-mouth disease (FMD) is an economically important global animal disease. To control FMD virus (FMDV) outbreaks, a lot of different novel approaches have been attempted. In this study, we proposed a novel porcine reproductive and respiratory syndrome virus (PRRSV) as a replicon vector to express FMDV structural protein.
Materials and Methods: PRRSV infectious clone (PRRSVK418DM) was used to develop an expression vector through the reverse genetic manipulation of PRRSV; FMDVP12A3C gene of serotype O was synthesized and used for an antigen. MARC-145 cells (African green monkey kidney epithelial cell line) were used for electroporation mediated transfection. The transfection or the expression of P12A3C and N protein of PRRSV was analyzed by either replicon containing PRRSV alone or by co-infection of helper PRRSV.
Results: We constructed PRRSVK418DM replicon vector containing FMDVP12A3C, and genome sequences were confirmed by subsequent sequence analysis. In vitro expression of P12A3C and PRRSV N protein was confirmed by immunofluorescence antibody assay using antibodies specific for PRRSV N protein (anti-PRRSV N MAb), FMDV-VP1 (anti-VP1 MAb).
Conclusion: The results indicate that PRRSV replicon vector can be a promising novel vector system to control FMDV and useful for vaccine development in the future.
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KEYWORD
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Replicon, FMDV, PRRSV, Genetic vectors
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